Use of N-methyl-N-nitrosourea treatment of fertilized egg cells for saturation mutagenesis of rice
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15 صفحه اولSome effects of treatment of hen erythrocytes in vitro with N-methyl-N-nitrosourea.
Studies have been made of the effect of N-methyl-N-nitrosourea on hen erythrocytes in vitro. These were done to find whether the highly condensed state of the chromatin and the very low metabolic activity of these cells would affect the extent of methylation of the DNA and chromatin proteins and the persistence of any methylation sites in these macromolecules with time after treatment. Also, th...
متن کاملN-ethyl-N-nitrosourea mutagenesis: boarding the mouse mutant express.
In the mouse, random mutagenesis with N-ethyl-N-nitrosourea (ENU) has been used since the 1970s in forward mutagenesis screens. However, only in the last decade has ENU mutagenesis been harnessed to generate a myriad of new mouse mutations in large-scale genetic screens and focused, smaller efforts. The development of additional genetic tools, such as balancer chromosomes, refinements in geneti...
متن کاملNon-phenotypic selection of N-methyl-N-nitrosourea-induced mutations in human cells.
The distribution of mutations in a particular gene as detected by a selective mutation assay could be affected by the structural properties of the target protein. To investigate this, we have analysed N-methyl-N-nitrosourea (MNU)-induced mutations in two restriction recognition sequences of a target gene for mutation analysis and compared these data with what previously observed in a phenotypic...
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ژورنال
عنوان ژورنال: Breeding Science
سال: 2010
ISSN: 1344-7610,1347-3735
DOI: 10.1270/jsbbs.60.475